Platelet-rich plasma has been advocated in the medical world for a number of benefits that it possesses. It has been well researched that the Platelet Rich Plasma has promoted Healing stimulation for over 25 years. The reason for such a useful medical purpose is that platelets, when activated, releases bioactive proteins, including growth factors and cytokines, that ultimately enhances the repairing and the regenerative processes. A gentle centrifugation, originating from the peripheral blood, purifies the platelet-rich plasma.
And the best thing about this process is that, due to the autologous origin, it is safe, as well as cheap, easy and fast. The uses of platelet-rich plasma are vast, nonetheless. It is preferred in a number of medical fields, which includes rheumatology, regenerative sports medicine, orthopaedic and plastic surgery. All these have shown to bear remarkable results.
Over the years, a number of research and clinical trials have been conducted to determine the numerous techniques available in the medical world in terms of the formulation of platelet-rich plasma. The preparations may include centrifugation speed and the use of an anticoagulant; the content includes, platelets, leucocytes and other growth factors; and then comes application. However, it must be noted that there is no particular standardized process to collect platelet-rich plasma. It has been found out that different method of preparation yields a different concentration of platelets. Even more, each preparation has the ability to produce different quality of PRP for different applications. Here is a description of the formulation of PRP.
Donors are selected on the basis of no relevant disease and no drug use. Citrate anticoagulant is used to maintain the integrity of the platelet. ACD-A is the most preferred and the mostly used anticoagulant for platelet-rich plasma preparation. This is because ACD-A has shown better maintenance power for the intraplatelet signal transduction mechanisms in the process of PRP preparation. This improves the responsiveness of the platelets, during the formulation. However, ethylenediaminetetraacetic acid anticoagulant is also used to determine the number and concentration of the platelet, using an automatic cell counter.
A standard laboratory centrifuge is used to conduct a soft spin on the sample. This PRP process helps to collect two different phases - one clouded phase comprising of platelets, platelet-poor plasma and buffy coat; the other phase contains red blood cells, at a ratio of 45% AND 55%. After several steps of purification and concentration, a controlled cellular therapy product is obtained, which is ideal for treating human medical conditions.
Platelet-rich plasma samples are collected and tested for various growth factors including, platelet-derived growth factor (PDGF-AB), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-1) and transforming growth factor (TGF-β1). These are conducted with the help of enzyme-linked immunosorbent assay (ELISA) kits (R&D Diagnostics).
After the collection, pH level of the platelet-rich plasma is checked using a litmus paper. Following this, the PRP goes through the stages of aggregometry, thromboelastography, electron microscopy and the platelet-rich plasma is tested for stability. Dr Vineeth MB, Regenerative Sports Medicine Specialist in Kochi does PRP process and PRP Preparation in Ernakulam. prp test with thromboelastography, electron microscopy in Kerala, India
